While, the formulations containing 50 % unsaturated long chain fatty acids showed considerable drug degradation as well as significant discoloration. Accordingly, The formulations containing 100 % saturated medium chain glycerides provide excellent chemical and physical stability pattern and have the potential to provide a stable dosage form of cinnarizine.”
“Aims
The KIT receptor is considered
as a reliable marker for a subpopulation of interstitial cells (IC), and by persistent neonatal inhibition of KIT we have investigated the role of this receptor in the development NU7441 in vivo of IC-networks in bladder and we have observed the functional consequences of this inhibition.
Methods
Newborn
rat pups were treated daily with the KIT inhibitor imatinib mesylate (IM). After 7 days animals GDC 941 were sacrificed and bladder samples were dissected for morphological and functional studies. Morphological research consisted of immunohistochemistry with IC specific antigens (KIT and vimentin) and electron microscopy. The functional studies were based on isolated bladder strips in organ baths, in which spontaneous bladder contractility and the response to a non-subtype selective muscarinic agonist was evaluated.
Results
Suburothelial and intramuscular IC were found and characterized in neonatal rat bladder. IM-treatment induced a significant selleck products decrease in numbers of IC based on specific immunohistochemical
markers, and electron microscopy revealed evidence of IC cell injury. These morphological alterations were observed on intramuscular IC only and not on IC in the suburothelium. Isolated muscle strips from IM-treated animals had a lower contractile frequency and an altered response to muscarinic agonists.
Conclusions
The present study shows the presence of regional subpopulations of IC in neonatal rat bladder, provides evidence for a dependence on KIT of the development of intramuscular IC and supports the hypothesis that a poor development of networks of intramuscular IC might have repercussions on spontaneous and muscarinic-induced bladder contractility. Neurourol. Urodynam. 33:461-468, 2014. (c) 2013 Wiley Periodicals, Inc.”
“The aim of the present study was to establish a method for the determination of folic acid and related substances content in dispersible tablets. Method: A Shim-pack VP-ODS C-18 reversed phase column (4.6 mm X 250 mm) was used. The mobile phase consisted of methanol-phosphate buffer (20:80) with a pH of 6.3. The flow rate was 1.0 mL/min, and the detection wavelength was 277nm and the column temperature was 30 degrees C. Results: The calibration curve was linear in the range of 5 similar to 150 mu g/mL (r = 0.9998) for folic acid. The minimal detection limit was 99.08 %, n = 9 and the related substances were well separated.