The fecal

The fecal e-book specimen was preserved at -80��C after collection. Strain ph1T (Table 1) was isolated in 2011 by anaerobic cultivation on 5% sheep blood-enriched Columbia agar (BioMerieux, Marcy l��Etoile, France) after 26 days of preincubation of the stool sample in an anaerobic blood culture bottle enriched with sterile blood and rumen fluid. Table 1 Classification and general features of Peptoniphilus obesi strain ph1T according to the MIGS recommendations [24] This strain exhibited a 91.0% nucleotide sequence similarity with P. asaccharolyticus and P. indolicus, the phylogenetically closest validated Peptoniphilus species (Figure 1). Among the validly published Peptoniphilus species, the percentage of 16S rRNA sequence similarity ranges from 86.0% (P. ivoriivs. P. olsenii) to 98.5% (P.

asaccharolyticus vs. P. indolicus). Despite the fact that strain ph1 exhibited a 16SrRNA sequence similarity lower than the 95.0% cutoff, which is usually regarded as a threshold for the creation of new genus [2], we considered it as a new species within the Peptoniphilus genus. Figure 1 Phylogenetic tree highlighting the position of Peptoniphilus obesi strain ph1T relative to a selection of type strains of validly published species of Peptoniphilus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using … Different growth temperatures (25, 30, 37, 45��C) were tested. Growth was observed between 30��C and 45��C, with optimal growth at 37��C. Colonies stained gray, transparent, opaque, non-bright and were 0.4 mm in diameter on blood-enriched Columbia agar.

Growth of the strain was tested under anaerobic and microaerophilic conditions using GENbag anaer and GENbag microaer systems, respectively (BioM��rieux), and in the presence of air, with or without 5% CO2. Optimal growth was achieved anaerobically, but no growth occurred in microaerophilic or aerobic conditions. A motility test was negative. Cells grown on agar are Gram-positive (Figure 2) and diameter ranged from 0.77��m to 0.93 ��m with a mean diameter of 0.87 ��m by electron microscopy (Figure 3). Figure 2 Gram staining of P. obesi strain ph1T Figure 3 Transmission electron microscopy of P. obesi strain ph1T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 200 nm. Strain ph1T exhibited neither catalase nor oxidase activities. Using the API rapid ID 32A system (BioM��rieux), positive reactions were observed for arginine Entinostat arylamidase and leucine arylamidase.

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