Conclusion On the basis of phenotypic, phylogenetic and genomic <

Conclusion On the basis of phenotypic, phylogenetic and genomic selleck chem analyses, we formally propose the creation of Kurthia massiliensis sp. nov., which contains the strain JC30T. This bacterium was found in Senegal. Description of Kurthia massiliensis sp. nov. Kurthia massiliensis (mas.si.li.en’sis. L. masc. adj. massiliensis of Massilia, the old Roman name for Marseille, where the type strain was isolated). Isolated from stool of a healthy Senegalese patient. K massiliensis are aerobic Gram-positive coccobacilli. On sheep blood agar after 24 h aerobic incubation at 37��C, colonies of strain JC30T are circular, greyish/yellowish, shiny, curved and smooth, 2-5 mm in diameter. Cells are motile by peritrichous flagella and encapsulated. Catalase activity is positive but oxidase activity is negative.

Gelatine hydrolysis, maltose assimilation, potassium gluconate assimilation, malic acid assimilation, trisodium citrate assimilation are present. Esterase (C4), esterase lipase (C8), cystine arylaminidase, ��-gluconidase activities are observed. Valine arylaminidase and alpha-chemotrypsin activities are weakly positive. The major fatty acids are iso C15:0 68.04% and anteiso C15:0 16.92%. Polar lipids found are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phospholipid 1. The peptidoglycan type is A4��L-Lys��D-Glu (type A11.33 according to [36]). Cells are susceptible to penicillin G, amoxicillin, amoxicillin + clavulanic acid, imipenem, gentamycin, erythromycin, doxycycline, rifampicin, vancomycin and nitrofurantoin. The G+C content of the genome is 39.

26%. The type strain is JC30T (= CSUR P141T = DSM 24639T). Notes Abbreviations EMBL European Molecular Biology Laboratory
Huanglongbing (HLB), also known as citrus greening, is a disease that poses a major economic threat to the worldwide citrus industry [1,2]. The disease was discovered to be present in Florida in 2005 and is characterized by yellowing of citrus tree leaves, premature defoliation, small bitter fruit, and a pale green fruit color after ripening. No known cure for the disease has been discovered, but preventative measures include chemical treatment against insect vectors and removal of infected trees to prevent the spread of disease [3,4]. The causal agents of HLB are believed to be Candidatus Liberibacter asiaticus, Candidatus L. africanus, and Candidatus L.

americanus, named according to the regions of where the organism was first identified [5,6]. Similar diseases have been found to occur in potatoes (Solanum tuberosum) and other solanaceous crops infected with Candidatus L. solanacearum [7]. Additionally, the Liberibacter genus contains the plant endophyte Candidatus L. europaeus Brefeldin_A [8], signifying that virulence in the Liberibacter genus is not universal.

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