The authors express their thanks to all the members of the Malaysian Organization of Pharmaceutical Industries for their voluntary participation in this study. “
“Acute ischemic stroke is a leading death cause worldwide.1 Stroke survivors struggle with serious disabilities, including paralysis, speech and/or language
problems, loss of balance or coordination, and memory loss. Several pathological processes involved in ischemia includes oxidative stress, inflammation, excitotoxicity, calcium www.selleckchem.com/products/BI6727-Volasertib.html overload, distraction of blood brain barrier and platelet activation and nitric oxide release.2 Oxidative stress is an important event to generate free radicals which can further demand tissue apoptosis. Therefore a potent anti-oxidant intervention may be beneficial in the treatment of cerebral ischemia and reperfusion injury. Recent investigations have been shown that the antioxidant properties of plants could be correlated with oxidative stress defense and different
human diseases including cancer, atherosclerosis and the aging process.3 and 4 The anti-oxidants can interfere with the oxidation process by reacting with free radicals, INCB018424 clinical trial chelating free catalytic metals and also by acting as oxygen scavengers.5 Among the plants known for medicinal value, the plants of the genus Coleus belonging to the family Lamiaceae or Labiatae are well known for their therapeutic potentials. The plants of Lamiaceae are usually aromatic and known for kitchen herbs like Rosemary, Ocimum sanctum, and Oregano. Many of the plants of this family are used in traditional medicine because of their antimicrobial, antioxidant, also antiseptic and other pharmacological activities. 6 However properties in different species of Coleus were little known. By this virtue of literature we focused to investigate the effect of aqueous root extract of Coleus edulies (ACE) on cerebral ischemia induced oxidative stress. Earlier reports suggested that anti-oxidants have potential role in treating ischemia. 7 and 2 Cerebral ischemia and reperfusion model employed
in the present study has been reported to simulate the clinical condition of cerebral ischemia in humans. 8 Hence the present study was an attempt to investigate the possible protective role of ACE in cerebral ischemia and reperfusion injury in rats. Thiopentone sodium (Neon-labs, Mumbai), 2,3,4-tetrazolium chloride (National Chemicals, Vadodara), Thiobarbituric acid (Sigma–Aldrich India), 1,1,3,3-tetraethoxy-propane (Sigma–Aldrich India), nitroblue tetrazolium (Sigma–Aldrich India), Nicotinamide adenine dinucleotide phosphate reduced form (Sigma–Aldrich India), Aqueous extract of coleus edulis (Laila implex, Vijayawada), All other chemicals and reagents used were analytical grade. Adult Wistar rats (220–310 g) were obtained from the Gentox Bio Pvt. Ltd., Hyderabad, Andhrapradesh, India. Animals were maintained under a 12/12-h light/dark cycle, in an ambient temperature (24 ± 1 °C) colony room.