The residual FVIII activity
was determined at the time of the 1rst week of treatment. Plasma of offspring from FVIII-treated mothers (BM/FVIII, closed circles) and from PBS-treated mothers (BM/PBS, opened circles) was recovered 30 min after the injection of 1 IU FVIII. A chromogenic assay was performed to measure the residual BMN 673 concentration FVIII activity in plasma. Figure S2. Theoretical and experimental clearance rates of maternal anti-FVIII IgG titers in the circulation of the progeny. The theoretical clearance rate of circulating maternal anti-FVIII IgG in the blood of B/FVIIIM/FVIII (grey circles) and B/PBSM/FVIII (grey squares) was calculated based on the reported half-life of mouse IgG (7 days)10,11 and on the initial titers measured in the serum 7 weeks after birth (Pre-treatment levels for B/FVIIIM/FVIII [212.8 μg/mL] and B/PBSM/FVIII [141.5 μg/mL] Figure 3A). The experimental levels of residual anti-FVIII IgG are reported
in the case of B/FVIIIM/FVIII mice HIF inhibitor (filled circles) and B/PBSM/FVIII mice (open squares) at 7 weeks of age, at the time of the 3rd injection and at the time of the 4th injection (data from Figure 3B). “
“We evaluated inflammatory markers in febrile neutropenic lymphoma patients undergoing high-dose chemotherapy with autologous stem cell support. Based on MASCC scores, our patients had a low risk of serious complications and a perspective of a benign initial clinical course of the febrile neutropenia. We also studied the impact of tobramycin given once versus three times daily on these immune markers. Sixty-one patients participating in a Norwegian multicentre prospective randomized clinical trial, comparing tobramycin once daily versus three times daily, given with cAMP penicillin G to febrile neutropenic patients, constituted a clinically homogenous group.
Four patients had bacteraemia, all isolates being Gram-positive. Thirty-two patients received tobramycin once daily, and 29 patients received tobramycin three times daily. Blood samples were taken at the onset of febrile neutropenia and 1–2 days later. All samples were frozen at −70 °C and analysed at the end of the clinical trial for C-reactive protein (CRP), procalcitonin (PCT), complement activation products, mannose-binding lectin (MBL) and 17 cytokines. We found a mild proinflammatory response in this series of patients. CRP was non-specifically elevated. Ten patients with decreased MBL levels showed the same mild clinical and proinflammatory response. Patients receiving tobramycin once daily showed a more pronounced proinflammatory response compared with patients receiving tobramycin three times daily. Overall, febrile neutropenic cancer patients with a benign clinical course show a mild proinflammatory immune response.