The reduced amount of three BAI genes in glioma tissues might be used as one of the molecular markers for that prediction of high quality glioma. Caspase3 activity without addition of cytochrome c was used as the negative control. Government method of D galactosamine and tea catechin derivatives in rats. Liver apoptosis was induced in accordance with Muntanes method, by intraperitoneal injection of N galactosamine. An individual dose of D galactosamine was administered intraperitoneally, and mice were sacrificed 12 h after the treatment. Two doses of EGCG with 50 lg/kg of LPS were given intraperitoneally Clindamycin ic50 at 1 h before and after the D galactosamine administration. EGCG was more given twice at 3 h intervals. Liver cytoplasm portion for caspase 3 activity analysis was prepared by sequential centrifugation method for cell organelle separation based on a described by Fleisher and Kervina. assay. Apoptotic cells were found in sections using the in situ Apoptosis Kit. Frozen parts of liver cells were fixed in 3% paraformaldehyde, incubated with protease K for 10 min, and then presoaked in terminal transferase buffer for 10 min. Sections were incubated for 1 h at 3-7 C in 2-5 ml of TdT option, containing 1 final transferase buffer, 0. 5 nM of biotin dUTP, and Endosymbiotic theory 10 U of TdT. After the TdT response, pieces were soaked in TdT stopping load, incubated with HRP conjugated streptavidin for 30 min at room temperature, and developed for 10 min in phosphate buffered citrate containing 0. 6 mg/ml DAB. Nuclei were counterstained with hematoxylin. 3. 1. Being an molecule of apoptosis within the final step of various apoptotic cascades inhibition of caspase 3 exercise by various catechin derivatives in-vitro Caspase 3 plays a key position. Caspase 3 activity was com-pletely inhibited by EGCG at 110 7 M and was inhibited to 50% at 110 8 M in vitro. Epi catechin gallate showed 50-year inhibition at 110 7M, and catechin gallate, epi catechin and epigallo catechin had caused inhibition at 110 6 M. Catechin, gallo catechin and gallate confirmed no inhibition as Dining table 1 shows. The music binding form of OH to the catechin band must be an epi structure to produce inhibitory CTEP activity. The current presence of either component, catechin gallate and/or ep-i form catechin, is important. Relationship of rate and substrate concentration of caspase3 in-the pres-ence of EGCG showed the Lineweaver Burk relationship and a sigmoidal curve did not give a line, but showed a logarithmic curve. The logarithmic curve changed to a straight line, as 1/ 2 once the abscissa was taken. The kinetics of these catechin derivatives appear to be a second purchase sigmoidal allosteric inhibition as follows: 1 vKm V e12T t 1 V.