Such tissues can rapidly form stable structures during inflammation, and yet equally as easily regress, as seen in the dynamic development of TLOs during chronic Helicobacter pylori infection.[57] The fundamentals underpinning SLO development also lie at the heart of TLO development: inflammatory cytokine expression (LT/tumour necrosis factor-α); stromal activation and chemokine production; and high endothelial venule development.[58, 59] As seen in transplantation studies,[60,

61] activated stromal cells alone are capable of initiating TLO formation in some instances, indicating their overriding capacity to contribute to TLO development. Nevertheless, the precise signals leading to stromal activation

during buy Ensartinib TLO development in vivo are still unclear. The majority of mechanistic data on the development of TLOs are CHIR-99021 nmr derived from transgenic mice expressing molecules in ectopic sites. Although these are narrow models that lack the complexity that undoubtedly underpins in vivo TLO generation, they do offer a glimpse into TLO development that would otherwise be hard to observe. Table 2 highlights animal models of TLO development that use either LTβR signalling, homeostatic chemokine or non-homeostatic cytokine transgenic expression. If TLO and SLO development is conceptually similar, what is the source of LTα1β2 in TLO development? One possibility is that TLOs are formed by LTis in much the same way as in SLOs, but there is conflicting evidence to support this hypothesis. Interleukin-7 (a key survival factor for LTis in developing SLOs) transgenic mice develop a large number of LNs and Peyer’s patches, as well as the formation of organized TLOs after immunization with antigen, in a process that is dependent upon LTα1β2 and the LTi-associated transcription Metformin cost factor retinoic

acid-related orphan receptor γt (RORγt).[62] However, a CCL21 transgenic model of TLO development lacking LTis still develops TLOs,[63] with CD3+ CD4+ T cells the first to arrive at the site of TLO development, indicating an LTi-independent mechanism that may be unique to TLOs. Formation of TLOs during inflammation of the intestine is able to occur in the absence of RORγt (and hence LTis),[64, 65] although with the recent identification of multiple innate lymphoid cell (ILC) populations, which express similar levels of LTα1β2 to their LTi cousins,[66, 67] the extent to which RORγt-independent ILCs can contribute to intestinal TLO generation requires further investigation.[68] As B and T cells both express LTα1β2,[69] are relatively much more abundant in chronically inflamed tissues than LTis (or other ILCs), and activated conventional lymphocytes are known to play a role in TLO generation in the skin,[60] it is likely that B and T cells contribute significantly to TLO development during inflammation.