Equivalent for the dermal fibroblasts, most MMP mRNAs tested have been elevated in RA and OA fibroblasts in response to S. aureus components. Levels of substantially ele vated MMPs are shown in Figure 3a,b. The MMP mRNA expression pattern in response to IL 1 TNF and S. aureus lysate was comparable in RA and OA fibroblasts as well. Interestingly, no considerable differences were noted in MMP 13 mRNA levels amongst the treated and untreated fibroblasts. All other MMPs tested were expressed at quite low levels, could not be quanti fied, and hence have been not integrated within the graph. Two far more dermal fibroblast lines, RASF and OASF cell lines, have been tested for many MMP mRNA expression profile upon exposure to S. aureus culture supernatants and bacterial cell lysates.
Essentially precisely the same profile as described above was obtained from the more cell lines. For the reason that fibroblasts are heterogeneous in terms of their origin and some of their capabilities, it is probably that fibroblasts from dif ferent sources may respond slightly differently when it comes to MMP expression. kinase inhibitor Potentiation of MMP protein expression in human fibroblasts by S. aureus grown in presence of rhIL 1 We’ve observed significant changes in gene expression in S. aureus grown inside the presence of rhIL 1.To test whether S. aureus grown in the presence of rhIL 1 would have any impact on MMP expression, dermal fibroblasts were exposed to 25g ml per effectively bacterial cell lysate obtained from S. aureus grown inside the presence of five or 15 ng ml rhIL 1.The supernatants have been collected and expression of various MMP protein was assessed by multi MMP Array kit from RayBiotech, Inc, as described previously.
The data presented in Figure four show that production of MMP two, 3, and eight is higher in fibroblasts treated with cell lysate obtained in the S. aureus strain grown inside the presence of rhIL 1.TIMP four expression was also slightly enhanced in fibroblasts treated with lysate obtained in the recommended site S. aureus grown in the presence of 15 ng ml IL 1.Induction of MMP mRNA in human dermal fibroblasts by fractionated culture supernatants from S. aureus The MMP inducing active elements within the culture superna tants were largely inside the 30 to 50 and 50 kDa molecular weight range as evidenced by drastically elevated expres sion of MMP 1 and MMP three by Centricon fractions 30 to 50 and 50 kDa in dermal fibroblasts. Although the fractions are not identified beyond their molecu lar weight, this does rule out a number of the currently characterized low molecular weight extracellular items of S. aureus. MMP mRNA induction by Sar, Agr, and Sar Agr mutants of S.