Microbiology 2003, 149:167–176.CrossRefPubMed 37. Struve C, Krogfelt KA: Role of capsule in Klebsiella pneumoniae virulence: lack of correlation

between in vitro and in vivo studies. FEMS Microbiol Lett 2003, 218:149–154.CrossRefPubMed 38. Sahly H, Keisari Y, Crouch E, Sharon N, Ofek I: Recognition of bacterial surface polysaccharides by lectins of the innate immune system and its contribution to defense GSK2118436 purchase against infection: the case of pulmonary pathogens. Infect Immun 2008, 76:1322–1332.CrossRefPubMed 39. de Astorza B, Cortés G, Crespí C, Saus C, Rojo JM, Albertí S: C3 promotes clearance of Klebsiella pneumoniae by A549 epithelial cells. Infect Immun 2004, 72:1767–1774.CrossRefPubMed 40. Greenberger MJ, Kunkel SL, Strieter RM, Lukacs NW, Bramson J, Gauldie J, Graham FL, Hitt M, Danforth JM, Standiford TJ: IL-12 gene therapy protects mice in Nirogacestat lethal Klebsiella pneumonia. J Immunol 1996, 157:3006–3012.PubMed 41. Standiford TJ, Wilkowski JM, Sisson TH, Hattori N, Mehrad B, Bucknell KA, Moore TA: Intrapulmonary tumor necrosis factor gene therapy increases bacterial clearance and survival in murine gram-negative Stattic pneumonia. Hum Gene Ther 1999, 10:899–909.CrossRefPubMed 42. Ye P, Garvey PB, Zhang P, Nelson S, Bagby G, Summer WR, Schwarzenberger P,

Shellito JE, Kolls JK: Interleukin-17 and lung host defense against Klebsiella pneumoniae infection. Am J Respir Cell Mol Biol 2001, 25:335–340.PubMed Authors’ contributions VC carried out the experiments involving lung epithelial cells infections. DM and ELL carried out the animal experiments. JAB. and JG conceived the study and wrote the manuscript. All authors read and approved the final version of the manuscript.”
“Background Laboratory contamination can be defined as the inadvertent addition of analytes to test samples during sample collection, transportation or analysis. There is a high level of awareness of the potential for cross contamination

when using nucleic acid amplification methods Dapagliflozin [1]. Although conventional microbial culture also represents amplification of signal to detectable levels there is relatively little systematic data on the frequency of cross contamination in conventional microbiology. In clinical laboratories cross contamination can lead to misdiagnosis of patients, inappropriate treatment or isolation of patients and investigation of pseudo-outbreaks. Detection of pathogens in food items can lead to very significant economic loss [2] therefore it is important to ensure that positive results reflect true product contamination. Sources of microbial laboratory contamination may include positive control strains, cultures of recent isolates, laboratory workers and airborne exogenous material such as fungal spores.