Key Word(s): 1. IBS; 2. SIBO; 3. Rome III criteria; 4. GHBT; Presenting Author: TIAN XIA Additional Authors: XIAOMING JIANG, YONGFU SHAO, BINGXIU XIAO, JUNMING GUO Corresponding Author: JUNMING GUO Affiliations: Ningbo University Objective: Long nocoding RNAs (lncRNAs) play important regulatory roles in cellular biology. Several studies showed that lncRNAs can be function as competing endogenous RNAs (ceRNAs). However, further work is required to understand the functions of ceRNAs in normal and pathological conditions. Methods: To measure the function of microRNAs (miRNAs) on lncRNAs

expression, we transfected miRNA mimics into gastric cancer cell lines. We constructed a ceRNA network mediated by miRNAs based on lncRNA microarray data and bioinformatic algorithms including miRcode and TarBase. Results: MiRNAs suppressed lncRNAs abundance. For instance, miR-129–5p Obeticholic Acid suppressed lncRNA AC130710.1 selleck screening library in MGC-803 cells. We screened lncRNAs which aberrantly expressed in gastric cancer tissues. Our analysis showed a ceRNA network including lncRNAs and mRNAs in gastric cancer. Eight lncRNAs and nine miRNAs participate in the ceRNA network. These lncRNAs regulate mRNAs (e. g., CDKN1A, E2F1, PTEN, RB1, RUNX1, and VEGFA) expression by using miRNA response elements (MREs) to compete for the binding of the shared miRNAs. Conclusion: Our study suggested that lncRNAs harbor

MREs and participate in a complex ceRNA network. The network brings to light an unknown miRNA regulatory network in gastric cancer, and suggests lncRNAs may play regulatory roles in post-transcriptional regulation. Key Word(s): 1. Long noncoding RNAs; 2. ceRNA network; 3. microRNAs; 4. gastric cancer; Presenting Author: WUQI FANG Additional Authors: CAICHANG CHUN Corresponding Author: CAICHANG CHUN Affiliations: university of jiujiang Objective: Gastric adenomyoma (AM) is a rare, benign tumor, characterized by gland-like structures embedded within a smooth muscle stroma. Methods: Here, we report a case of a 55-year-old woman with gastric AM admitted to our hospital for upper abdominal pain. Endoscopic

examination revealed a gastric mass of about 1.5 cm in diameter, located in the junction this website of gastric body, which was very rare. The surface of the mass was smooth, but erosion at the top. Carbohydrate antigen 125 was 141 U/mL, carcinoembryonic antigen, cancer antigen 19–9, alpha fetoprotein and hemoglobin were within the normal range. Results: The examination of fecal occult blood was positive. Abdominal computed tomography (CT) scanning showed a small amount of ascites, which was difficult to obbtain for examination, the gastric wall, liver or lymph nodes were not observed abnormalities. Then we reset the mass with submucosal ligation and suck endoscopic separated resection (SLSER) (Figure 1A). The surgery removel specimen was yellowish-white. The histopathological examination revealed a gastric adenomyoma (Figure 1B).