James Miller in 1973 [76]. In this study Miller conducted an extended immunization regimen in rabbits, consisting of 60 intravenous injections of a total of 3.71 × 109 γ-irradiated T. pallidum over a 37-week period, followed by intradermal challenge of either 103 or 105 homologous Nichols strain T. pallidum. Immunized rabbits displayed complete protection, as demonstrated by the lack of development of chancres at the challenge sites and the absence of infection in naïve

recipient animals receiving lymph nodes from the immunized rabbits. Protection persisted for at least one year after the final immunization [76]. This study was groundbreaking in that it established proof-of-principle that complete protection from infection and disease could be achieved in the animal model, albeit through an immunization regimen that is not tenable Alpelisib solubility dmso in humans. Another critical facet of this study was Miller’s insightful recognition that the treponemal surface was responsible

for conferring the observed protection. Miller reasoned that failure of previous attempts to induce protection using T. pallidum inactivated by mechanical or chemical treatments [77], [78], [79], [80], [81] and [82] (see also detailed reviews in [83] and [84]) was due to the destruction of labile protective surface antigens. Although most investigators focus on the OMPs of T. pallidum, it must be remembered that much of the T. pallidum Gefitinib molecular weight surface is comprised of membrane lipids which induce the anti-lipoidal antibodies used to diagnose syphilis in patients with the VDRL and RPR tests. These lipid antigens were included in the immunogen used by Miller. Separate studies have shown that immunization of rabbits with this lipoidal antigen induces the production of opsonic antibodies and partial protection against infectious challenge [85]. Further, a highly-neutralizing monoclonal antibody derived following immunization of mice with intact T. pallidum was

shown to have specificity for a phosphorylcholine surface epitope of T. pallidum. Passive immunization with this antibody resulted in significant attenuation of infection [86]. Further, Miller showed that attainment of immunity using γ-irradiated, non-proliferating treponemes required an extended period of 37 weeks, almost with only partial and no immunity observed over 24- and 12-week immunization periods, respectively [76]. Miller’s study also confirmed previous observations that protective immunity against re-infection with homologous T. pallidum strains develops, albeit slowly, in the animal model. Complete protection against symptomatic homologous strain challenge develops only after 12 weeks of infection. If rabbits are cured of infection prior to that 12 week milestone, they can be symptomatically re-infected [87], [88], [89] and [90]. It is now speculated that the slow development of protective immunity to T. pallidum correlates with the unusual protein-poor surface of the bacterium.