26). During surgery a light decrease in hematocrit and hemoglobin concentration was observed in both groups, but intra-operative ATM Kinase Inhibitor cell line blood loss was similar. Also, the volume of crystalloid administered during anaesthesia was similar in both groups. Similarly, no statistical differences were observed regarding hemodynamic and respiratory parameters. None of the patients experienced adverse clinical events during their postoperative course. In all patients no TED was observed in the post-operative period and in a 2-yr follow-up. This is probably due to the

anti-thrombotic prophylaxis which was carried out for ethical reasons in all patients 24 hrs post surgery because intra-operative changes EPZ-6438 mouse of some pro-coagulant markers were observed. Lymph node metastases were detected in only 4 out of 45 patients with lymph node dissection (8.9%): one in the TIVA-TCI group and 3 in the BAL group (p = 0.32). Types of anaesthesia and prothrombotic markers Changes of prothrombotic markers associated with the use of different techniques of anesthesia are reported in Tables 3 and 4. In both TIVA-TCI and BAL patients a significant and continuous reduction in CB-839 datasheet Screen clotting time PT (given as percentage) was observed during post-surgery period

(T2) as compared to T0 (p = 0.001), while aPTT was shortened at T1 and then normalised on the first postoperative day (T2). Table 3 Changes of prothrombotic markers in patients with prostate cancer who underwent surgery with total intravenous anesthesia with target-controlled infusion (TIVA-TCI) before the induction of anaesthesia (T0), 1 hr post-surgery (T1) and 24 hrs post-surgery Clomifene (T2)   T0 T1 T2 P         T0 vs T1 T1 vs T2 T0 vs T2 Screen clotting time             – PT (%) 93.1 (1.3) 85.6 (1.2) 82.5 (1.2) 0.001

0.21 0.001 – PTT (sec) 29.6 (0.6) 26.8 (0.7) 27.6 (0.8) 0.003 0.07 0.18 Procoagulant markers             – Fibrinogen (mg/dL) 285.5 (7.1) 262.3 (6.6) 353.3 (8.8) 0.004 0.001 0.001 – TAT (ng/L) 9.1 (1.9) 22.8 (3.2) 9.7 (2.4) 0.002 0.004 0.79 – F1 + 2 (pmol/L) 210.8 (27.3) 622.1 (64.2) 364.4 (45.6) 0.001 0.001 0.007 – FVIII (%) 142.9 (8.1) 194.2 (9.3) 162.3 (5.6) 0.001 0.004 0.04 Fibrinolysis markers             – PAI-1 (ng/ml) 15.2 (1.4) 21.9 (5.8) 36.1 (9.8) 0.41 0.20 0.04 – D-dimer (μg/L) 127.1 (12.8) 721.4 (170.4) 364.2 (28.3) 0.001 0.02 0.001 Haemostatic system inhibitors             – AT (%) 102.1 (1.8) 90.6 (1.9) 87.4 (2.4) 0.001 0.38 0.001 – protein C (%) 109.6 (2.8) 95.4 (2.8) 87.8 (2.8) 0.004 0.03 0.001 – protein S (%) 93.8 (3.1) 84.2 (2.8) 82.4 (2.4) 0.01 0.56 0.001 Platelet-aggregating properties             – p-selectin (ng/ml) 37.9 (2.0) 36.8 (2.4) 33.5 (2.6) 0.78 0.37 0.28 Values are mean (SD).