By contrast, no differences in the percentage of CD8+ T cells sta

By contrast, no differences in the percentage of CD8+ T cells stained with antibodies directed

to IFN-γ, IL-4 and IL-13 were observed. Because CD8α+ DCs have been implicated as the main DC subset for cross-presentation and cross-priming of CD8+ T cells,21–23 we investigated whether treatment of allergic mice with OVA-pulsed DCHISs also resulted in the accumulation of CD8α+ DCs in the lungs. Figure 4(a,b) www.selleckchem.com/products/R788(Fostamatinib-disodium).html shows that i.t. injection of both OVA-pulsed control DCs and OVA-pulsed DCHISs resulted in a higher proportion of CD8α+ cells in the population of CD11c+ cells. However, the proportion of lung CD8α+ cells was significantly higher (P < 0·05) for mice treated with OVA-pulsed DCHISs versus OVA-pulsed control DCs. Moreover, we found that CD11c+ cells isolated from the lungs of mice treated with DCHISs released higher levels of LTB4 compared with CD11c+ cells isolated from the lungs of mice treated with control DCs (Fig. 4c). Because LTB4 displays a potent chemotactic effect on CD8

T cells,24 this result could explain the infiltration of the lungs by CD8+ T cells found in mice treated with DCHISs. We finally investigated whether administration selleck of OVA-pulsed DCs to allergic mice resulted in changes in serum levels of specific IgE antibodies or the percentages of eosinophils found in the BAL. In these experiments, OVA-pulsed DCs were injected 3 days after challenge of mice with aerosolized OVA, and BAL and serum samples Baricitinib were obtained 2 weeks later. Figure 5(a,b) shows that administration of OVA-pulsed DCHISs resulted in: (i) a significant increase in serum levels of specific IgE antibodies directed to OVA, and (ii) an increase of eosinophils percentages of eosinophils in BAL compared with mice treated with OVA-pulsed control DCs. Asthma is a complex respiratory disease characterized by persistent airway inflammation and AHR.25 Eosinophils, Th2 cells and mast cells play a critical role in asthma.26,27 These cells

are recruited in the lung and upon activation they release a number of cytokines and chemokines inducing airway inflammation. In contrast to the well-defined role of Th2 cells in the induction of IgE production, eosinophilia and AHR, the role of CD8+ T cells is less well established.28,29 A number of reports, however, have shown that CD8+ T cells are essential for the development of AHR and allergic inflammation.30 An increased number of CD8+ T cells were observed in the blood and in the BAL of asthmatic patients, while animal models of airway inflammation have revealed substantial CD8+ T-cell infiltration of the bronchial mucosa after allergic sensitization.

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