5 mm) www.selleckchem.com/products/dorsomorphin-2hcl.html and (b) transverse (x = 14.5 mm). Taking a further look at Figure 4a,b, with a working temperature of 25°C, the DNA molecule velocity had an electrophoretic velocity apparently of the same order of magnitude in both the y and z directions due to the uniformity across
the stream. The velocity was constant across the channel width (along the z direction) and height (along the y direction) with an increase from 60 to 125 μm/s as E x = 5 kV/m increased to 10 kV/m, as shown in Figure 4a,b. Tables 2 and 3 list the local velocity distributions measured at different electric strengths and heating temperatures, respectively. Table 2 Local velocity map (μm/s) with different heating temperatures y (μm) 25ºC 35ºC 45ºC 55ºC 10 62.51 93.40 124.45 61.55 94.23 123.59 62.55 95.88 124.79 63.89 95.46 126.97 0 62.23 93.33 124.09 61.83 93.53 123.57 62.22 94.29 125.06 63.74 94.89 126.57 −10 62.10
93.30 123.88 62.61 94.59 124.68 63.48 93.98 125.68 63.35 94.79 126.30 Error (%) 0.66 0.11 0.46 1.72 1.13 0.9 2.03 2.02 0.71 0.85 0.71 0.53 Velocity map at different heights of the channel in x = 14.5 mm and y = 10 to −10 μm. Table 3 Local velocity map (μm/s) with different heating temperatures and electric fields T ( C) 5 kV/m 7.5 kV/m 10 kV/m 25 62.23 62.31 62.52 93.33 93.44 93.53 124.09 124.05 124.20 35 61.83 62.45 62.56 93.53 93.55 93.60 123.57 123.78 123.94 45 62.22 62.33 62.54 94.29 93.88 93.90 125.06 124.99 125.15 55 63.74 63.54 63.60 94.89
94.67 94.75 126.57 126.41 126.43 Error (%) 3.10 1.97 1.73 1.67 1.32 1.30 2.42 2.12 2.01 Velocity map at different locations of the channel in x = 14.5, 14.6, and 14.7 mm and RG7420 y = 0. Figure 5 shows the relative velocity (|ΔV|; absolute value was taken), using a treated PDMS device to get the velocity of EOF of the buffer solution convection observed at four different temperatures, 25°C, 35°C, 45°C, and 55°C, for four corresponding heating powers at three electric strengths. Farnesyltransferase Convection rates were estimated by the increases in buffer solution temperature. Two different trends were observed: one (left half) at the same inlet position with different elevation and the other (right half) at the same elevation with a different downstream position. The former showed an irregular velocity |ΔV| distribution as the heating temperature increased at different electric strengths, while the latter exhibited a definite quadratic |ΔV| increase as the heating temperature increased. The significant influence of the buffer solution temperature increase on DNA molecule stretching was clearly noted. This was partly because the rise in temperature of the buffer governed the evaporation rate which would result in suspension deposited on the channel bottom surface, and consequently, it created the convective flux that dragged the DNA molecules and resulted in molecule stretching.