25 g/L. The mitotic index decreased with an increase in concentration of the extract. The extract induced chromosome aberrations which were statistically significant and dose-dependent compared to the negative control. (c) 2014 SAAB. Published by Elsevier B.V. All rights reserved.”
“Background/purpose:
Biofilm formation by Streptococcus mutans is a prerequisite for the development of caries. Different strains of S. mutans may differ in their capacity in biofilm formation and protein expression. The objective of this study was to investigate the morphological features PARP activity and proteomes of biofilms of S. mutans clinical isolates. Materials and methods: Clinical strains isolated
from caries-active (SM 593) and caries-free (SM 18) adults were cultured on polystyrene sheets in tryptone polypeptone yeast extract medium. Biofilm formation and structure were assessed by confocal laser scanning microscopy and scanning electron microscopy. Proteins were extracted from SM 593 and SM 18 presented in biofilms and separated with two-dimensional gel electrophoresis, followed by peptide mass fingerprinting using matrix-assisted laser desorption time-of-flight mass spectrometry analysis. Results: Initially detected 2 hours after incubation, biofilm formation reached its maximum level at 20 hours. The biofilm formed by SM 593 was thicker with a higher percentage of viable bacteria compared with that formed by SM 18. Hydrolase and pantothenate kinase were detected AC220 solubility dmso in the SM 593 biofilm only, whereas 6-pyruvoyl tetrahydropterin synthase and phosphoribosylglycinamide formyltransferase were expressed exclusively in the SM 18 biofilm. Flavopiridol cell line Expressions of D-alanyl-D-alanine carboxypeptidase and response regulator homolog of RumR were most greatly enhanced in the SM 593 and SM 18 biofilms, respectively. Conclusion: SM 593 exhibited greater biofilm-forming capacity compared to SM 18. SM 593 and SM 18 biofilms expressed specific proteins involved in nucleic acid metabolism and intermediary
metabolism, respectively, which may account for the differences in their biofilmforming abilities. Copyright (C) 2015, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. All rights reserved.”
“This review presents an overview of the most important rodent-borne hemorrhagic fever pathogens directly transmitted from rodents to humans, namely Leptospira and hantaviruses, together with the New- and Old-World arenaviruses. These zoonotic diseases frequently share clinical symptoms, transmission routes and other epidemiological features and often have an emerging pattern. Differential diagnostics could benefit from a syndrome-based approach grouping these pathogens.