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casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production. Vaccine 2005, 23:1335–42.PubMedCrossRef Authors’ contributions XQ carried out construction of expression plasmid, participated in the sequence alignment and drafted the manuscript. GL carried out the protein expression and immunoassays. XW and XL carried out the Immunizations. ML performed the statistical Oxaprozin analysis. YL conceived of the study, and participated in its design and coordination. All authors read and approved the final manuscript.”
“Background The Atlantic cod (Gadus morhua) is a cold-adapted fish species which has been captured for human consumption for many years. It is a perishable commodity and for that reason, preservation methods like freezing or salting have traditionally been used to extend its shelf life [1, 2]. The fish is a microbial ecosystem of its own where the ecological principles of succession are as valid as in any other ecosystem. This microbiological environment consists of a high nutrient content with an oxygen tension favourable to the proliferation of fast-growing heterotrophs also responsible for the spoilage of food [3–5].