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39. Cafiso V, Campanile F, Borbone S, Caia A, Cascone C, Santagati M, Stefani S:Correlation between methicillin-resistance CT99021 and resistance to fluoroquinolones in Staphylococcus aureus and Staphylococcus epidermidis.Infez Med2001,2:90–97. 40. Yang JA, Park DW, Sohn JW, Kim MJ:Novel PCR-restriction fragment length polymorphism analysis for rapid typing of staphylococcal cassette chromosome mec elements. J Clin Microbiol2006,44:236–238.CrossRefPubMed Authors’ contributions SD carried out the microbiological analysis of the samples, designed the primers and multiplex PCR conditions and drafted the manuscript. RA

FK506 solubility dmso assisted in the preparation of material and in the identification of the isolates. EJ and MLM participated in the characterization of the strains. RC set up and helped with the PFGE methodology. LF participated in the design of the study and performed the statistical analysis. JMR conceived of the study, coordinated it and revised the manuscript. All authors read and approved the final manuscript.”
“Background Calomys callosus (Rodentia-Cricetidae), a wild rodent, exists near farm residences in savannas and cattle breeding areas. It has been adapted to be bred in captivity under controlled laboratory conditions and values for reproductive parameters, such as age at reproduction, pregnancy time, number of litters, male/female ratio, growth curve, and some external anatomical values have also been determined [1, 2]. Laboratory inbred strain was obtained for experimental purpose [3, 4]. This rodent has been described as a reservoir of Trypanosoma cruzi, the causative agent of Chagas disease and of the

hantaviroses, zoonoses caused by the Bunyaviridae family [5, 6]. C. callosus naturally and experimentally infected with T. cruzi presents high parasitaemia values during the presumable first days of infection, Aurora Kinase which progressively decreases until becoming negative a few weeks later showing regression of the lesions within a few days [7]. The infection is accompanied by inflammation of both myocardium and skeletal muscle characterized initially by an infiltrate containing macrophages, fibroblasts and small numbers of lymphocytes. Although the mechanism underlying the resistance of C. callosus to T. cruzi infection is not totally understood, its ability to control and avoid tissue lesions might be a key factor involved in its resistance to pathogens [5, 6, 8, 9]. Nevertheless, when C.