0:1.0 of wall material:core material, and when they increased the concentration of core material, the yield declined. The low values obtained for encapsulation process

yield and encapsulation efficiency in the present Nintedanib study can be explained by the fact that the SPI showed low solubility, limiting its application in food systems, even after using ultrasound to improve the solubility of the protein (Mendanha et al., 2009; Molina-Ortiz et al., 2009). Another possible explanation is the fact that the pH was not altered in each trial to obtain stoichiometric equilibrium between the charges, and was hence distant from the ideal zeta potential. According to Jun-xia et al. (2011), a pH of 4.0 was the ideal value for systems formed from the biopolymers SPI:GA with a core material of orange essential oil, based on the encapsulation yield and absorbance of the supernatant observed in the pH range between 2.5 and 4.5. The analyses of the effects EPZ5676 price of the concentration of the wall materials (SPI:GA), the wall material to core material ratio (wall:core) and the TG concentration on the encapsulation efficiency, failed to present acceptable regression coefficients (R2 < 55%) for obtaining mathematical models considering the independent variables under study, even though the repeatability of the results was proven by the central point trials (C15, C16, C17 and C18–1.5:1.0 SPI:GA; 2.0:1.0

wall:core; 6.0 UA of TG/g), which did not present statistical differences between them (p > 0.05). In the comparison of the experimental trials with the control trials (C20 – without TG), the trial C14 (1.5:1.0 SPI:GA; 2.0:1.0 wall:core; 10 UA/g TG) showed the highest encapsulation efficiency of approximately 70 g/100 g, differing

statistically from the others (p < 0.05). Lamprecht et al. (2001) obtained lower results of 60% encapsulation efficiency for capsules of fish oil ethyl ester encapsulated in a matrix of gelatin:GA by complex coacervation, although they managed to get elevated results Molecular motor for encapsulation efficiency. The formation of microcapsules of fatty acid ethyl esters was confirmed by SEM. All the trials presented the same topographical conformation, with accentuated wrinkling and the presence of smaller microcapsules adhering to the surfaces, a characteristic better observed in Fig. 2. Tang and Li (2012) also observed indented and wrinkled surface morphology of microcapsules produced with SPI by spray drying process, very similar to those found in this work, and justified this fact by uneven shrinkage during the drying or the surface protein content of your wall. The presence of microcapsules with incomplete parts can be observed (C9), a fact also observed by Jun-xia et al. (2011), who indicated the need for more extensive studies in relation to the interaction of the two biopolymers used, since the presence of cracks could represent the non-sealing and incomplete formation of the microcapsules.