Previous authors have referred to this juxtaposition as: ��Apples are compared to oranges.�� . The reasons for this are partly because pHe measurement uses the same secondary measuring equipment (the glass pH electrode) and the same aqueous buffer solutions for calibration as its aqueous counterpart, but without a detailed understanding of the measurement being made , or with the traceability to the SI  that is available for aqueous pH measurements . The result of this situation is that the numerical value produced by pHe measurements are dependent on the standard method used, and the type of glass electrode employed.
To a certain extent, therefore, the presence of a detailed documentary standard method such as ASTM D6423 should provide measurements results with some limited stability and comparability (if not traceability or coherence) .
However a detailed investigation into the sensitivities of method to slight variations in key parameters such as measurement time, stirring rate, temperature, etc., has never been undertaken. This paper presents data describing empirically determined sensitivities of the current ASTM D6423 method, additionally allowing a more robust estimate of uncertainty of the procedures to be made, and furthermore makes suggestions to improve the reproducibility and repeatability of the standard method which might be considered during any future revision.
The authors Carfilzomib are not aware of any existing relevant literature examining the ASTM D6423 method, although discussion of the basis and issues surrounding for pH determination in non-aqueous solution  is available  and guidelines for non-aqueous pH measurement  have been published , although in general these do not deal with mixtures of ethanol mass fraction close to 1 .2.?Experimental SectionAll experimentation was conducted in a laboratory at 20 �� 2 ��C. All chemicals used were of high-purity grade (Fisher), buffers were of high accuracy (Fisher) and solution were prepared gravimetrically throughout using deionised water (Millipore, MilliQ). Borosilicate glass vessels were used throughout.
Prior to use these were Brefeldin_A thoroughly cleaned and washed and then rinsed with deionised water, before being filled with deionised water and left to stand for 48 hours to leach any remaining impurities adhered to the glass. The vessels were then rinsed again with deionised water and dried in an oven at 120 ��C. When required, temperature control was exerted by placing the measurement vessels in a thermostatic water bath. Measurements were made based on the procedure described in the standard method ASTM D6423 .