I review

a web of animal and human data that tightens the noose around the hypothesis that copper toxicity is causing the epidemic of Alzheimer’s disease and loss of cognition in our aging population.”
“The HLA-G (human leukocyte antigen-G) molecule plays a pivotal role in immune tolerance by inhibiting different cell subsets involved in both innate and adaptive immunity. Besides its primary function in maintaining the maternal-fetal tolerance, HLA-G has been involved in a wide range of pathological conditions where it can be either favorable or detrimental to the patient, depending on the nature of the pathology. Although several studies have demonstrated the utmost importance of the 30 untranslated region (3′UTR) in the HLA-G expression profile, limited data exist on the sequence variability of this gene Navitoclax research buy region in human populations. In this study, we characterized the genetic diversity and haplotype structure of the HLA-G 3′UTR by resequencing 444 individuals HM781-36B cost from three sub-Saharan African populations and retrieving data from the 1000 Genomes project and the literature. A total of

1936 individuals representing 21 worldwide populations were combined and jointly analyzed. Our data revealed a high level of nucleotide diversity, an excess of intermediate frequency variants and an extremely low population differentiation, strongly supporting a history of balancing selection at this locus. The 14-bp insertion/deletion polymorphism was further pointed out as the likely target of selection, emphasizing its potential role in the post-transcriptional regulation of HLA-G expression.”
“Aim of the study: The aim of the study was to evaluate the effectiveness of postoperative radiotherapy in prostate cancer patients with unfavorable prognostic factors.\n\nMaterial and

methods: In the years 2002-2008, 121 consecutive prostate Cilengitide cancer patients underwent radical prostatectomy and postoperative radiotherapy. The median dose was 64 Gy (range 60-72 Gy). Biochemical and clinical progression free survival were estimated. Univariate and multivariate analyses were used to analyze clinicopathological varibales associated with treatment failure.\n\nResults: The median follow-up was 27 months. Three-year bPFS was 72%. On univariate analysis it was influenced by extracapsular tumor extension (60% vs. 75%, p = 0.0232), seminal vesicles invasion (52% vs. 85%, p = 0.00041), Gleason score >= 7 (65% vs. 86%, p = 0.044) and the use of hormonal therapy (50% vs. 80%, p = 0.0058). On multivariate analysis bPFS was associated with: TNM stage (HR = 2.6), total irradiation dose (HR = 0.82) and the maximum pretreatment level of prostate-specific antigen (PSA) (HR = 0.95). Three-year cPFS was 84%. On univariate analysis it was influenced by preoperative PSA level > 10 ng/ml (75% vs. 90%, p = 0.04), vascular nerve bundles involvement (63% vs. 88%, p = 0.0031), adjacent organs infiltration (50% vs. 85%, p = 0.

“
“BACKGROUND: The Iowa Satisfaction with Anesthesia Scale BI-D1870 (ISAS) is a questionnaire that measures patient satisfaction with monitored anesthesia care. Previous assessments of the reliability and validity of this tool have been conducted in 2 separate single-center studies. Recently, the questionnaire was used in a 24-center, 315-patient, placebo-controlled trial of dexmedetomidine. We analyzed

the data from these patients to provide anesthesiologists and statisticians designing multicenter clinical trials the information needed to use the ISAS as a primary study end point.\n\nMETHODS: Trial variables were ISAS score, treatment group, center, time of administration of the instrument, and potential covariates of age, race, gender, type of surgery, and ASA physical status.\n\nRESULTS: The ISAS could be scored for 98% of patients (95% confidence interval [CI], >97%). Whereas 73% of patients responded with the minimum of -3 or the maximum of +3 for the single question “I was satisfied with my anesthetic care,” only 14%

did so for the ISAS (i.e., the instrument differentiated among patients). The internal consistency (Cronbach alpha) FRAX597 equaled 0.84 (95% CI, 0.79-0.87). Because there was no correlation between the ISAS score and time of completion of the questionnaire the next day (Kendall tau(b), 0.01; 95% CI, -0.06 to 0.09), the ISAS likely is valid when administered by telephone the next day, not only when administered in person upon discharge as shown in its original development. Effect sizes of 0.48

have been detected in several trials, suggesting that typically appropriate sample sizes are 70 to 93 patients per group. There was significant heterogeneity in ISAS scores among study centers (P < 0.0001), making stratification by center very important, and planned analysis with center as a covariate www.selleckchem.com/products/dibutyryl-camp-bucladesine.html reasonable. Type of surgery might be an additional appropriate covariate depending on the study.\n\nCONCLUSIONS: The ISAS is reliable, valid, and useful over the conditions suitable for use as a primary study end point in multicenter clinical trials. (Anesth Analg 2011;113:364-8)”
“Two high-resolution maps of meiotic recombination initiation sites across the genomes of budding yeast and mice illuminate broad similarities in the control of meiotic recombination in these diverse species but also highlight key differences. These studies offer new insights into the relationships between recombination, chromosome structure, and genome evolution.”
“Tissue-engineered scaffolds may improve experimental outcomes in cardiac cell therapy by targeted delivery of stem cells and mechanically support an infarcted left ventricular (LV) wall.

In a mixed condition, Participants and Observers read the reassurance and threat passage, respectively. Between-groups analyses revealed threat group participants had lower pain tolerance and reported less cognitive coping than did participants in other appraisal conditions. Threat group observers reported less attention diversion, coping self-statements and ignoring in helping their partner than did reassured

observers. Pain language was also most prominent in transactions of threatened dyads. Finally, use of attention diversion by observers contributed to pain tolerance, independent of participant factors (reported pain, appraisal condition, reported coping) and pain language in conversations during immersions. The study highlights how appraisal contributes not only to pain tolerance LB-100 manufacturer and coping in

the affected individual but also to care-giving efforts of others in their social environment. (c) 2008 European Federation of Chapters of the International Association for the Study of Pain. Published by Elsevier Ltd. All rights reserved.”
“Flow voids in the basal ganglia cannot always be recognized on magnetic resonance imaging, even in patients with typical moyamoya disease. In this report, flow voids in the basal ganglia and cisternal flow voids of the sylvian valley were evaluated in patients with moyamoya disease, and their diagnostic value was verified. A total of 41 consecutive patients with moyamoya disease were included in this analysis. The number of flow Bromosporine in vitro voids in the basal ganglia HIF-1 pathway and the sylvian valley were counted on each side by 3 observers. Then the numbers of flow voids were compared between the patients with moyamoya disease and controls. The patients with

moyamoya disease had a significantly higher mean number of flow voids in the basal ganglia and the sylvian valley (P < .0001); however, the number of flow voids in the basal ganglia was 0 or 1 in 69 sides (28.0%) in patients with moyamoya disease. Comparative analysis using the area under the receiver operating curve indicated that the evaluation of flow voids in the sylvian valley was significantly superior method to that in the basal ganglia (P < .0001). The cutoff value for the number of cisternal flow voids in the sylvian valley for the diagnosis of moyamoya disease was 6. Based on these findings, we recommend a definitive diagnosis of moyamoya disease should include assessment for abnormal vessels around the terminal portions of the internal carotid arteries.”
“The conversion of ethanol from paper sludge using the separate hydrolysis and fermentation (SHF) process with cellulase and Saccharomyces cerevisiae GIM-2 were investigated in this paper. Optimization strategy based on statistical experimental designs was employed to enhance degree of saccharification by enzymatic hydrolysis of paper sludge.

001). In addition, after reaching this minimum value, ruminal pH increased more slowly in this diet, inducing a decreased preprandial ruminal pH (P <

PF-03084014 nmr 0.001). Consequently, the ad libitum diet led to a longer time below pH 5.6. A slow decrease in ruminal pH may enable sheep to consume larger quantities of food. However, free access to concentrate maintains continuously elevated content of ruminal fermentation end products and so requires more time for pH to return to neutral values. Thus, interval between feed distributions should be as large as possible to help resume the preprandial ruminal pH and to limit time spent with pH < 5.6.”
“Hydrogen permeation through SrCe(1-x)Tb(x)O(3-delta) (x=0.025, 0.05 and 0.10) membranes using various gas streams as the sweep was investigated. Hydrogen impermeable SrCe(1-x)Tb(x)O(3-delta) membranes with air or inert gas in the downstream become hydrogen permeable when there is a reducing gas, such carbon monoxide or hydrogen, existing in the downstream. The membrane remains hydrogen permeable after the downstream buy Bafilomycin A1 sweep gas is changed from the reducing gas to

the inert gas. This phenomenon is explained by the electronic conductivity of the materials. These results further confirm that SrCe(1-x)Tb(x)O(3-delta) (0.025 < x < 0.1) is a mixed proton-electron conducting material in a hydrogen containing atmosphere. The activation energy of hydrogen permeation is close to the activation energy of electronic conduction of the materials,

confirming that the hydrogen permeation is determined by the electronic conductivity of the material. For SrCe(0.95)Tb(0.05)O(3-delta), increasing the downstream CO partial pressure from 0.001 to 0.1 atm leads to a small increase in hydrogen flux from 1.4 x 10(-2) to 1.6 x 10(-2) ml/cm(2) min. The hydrogen flux of SrCe(1-x)Tb(x)O(3-delta) increases QNZ mw with upstream hydrogen partial pressure. (C) 2009 Elsevier B.V. All rights reserved.”
“Background: Different decoctions of Alchornea cordifolia leaves are used by Yoruba herbalists (Southwest Nigeria) for the local treatment of ulcers, rheumatic pains, febrile convulsions, and for enhancing physical performance. Materials and methods: In this study, the anti-arthritic effect of 100 – 400 mg/kg/day of the hydroethanolic leaf extract of Alchornea cordifolia (HEAC) was investigated in Complete Freund’s Adjuvant (CFA)-induced arthritic rats as a way of evaluating its efficacy in the local management of arthritis. In addition, the effects of HEAC on liver and renal function parameters as well as its effect on the antioxidant enzyme system were investigated. Arthritis was induced using 0.1 ml of 10 mg/ml of Complete Freund’s Adjuvant (CFA) following 1 h oral pretreatment and 8th day post-arthritic induction with 100, 200 and 400 mg/kg/day of HEAC and 3 mg/kg/day of celecoxib as the reference drug.

001 and p smaller than 0.05, respectively), while levels of IL-10, MCP-1 and MIP-1 alpha were not significantly influenced by nephrectomy or voluntary exercise (p bigger than 0.05). Exercise was an effective non-pharmacologic approach to slow cardiovascular disease in the presence of kidney disease in the apolipoprotein E knockout mouse.”
“On Bonaire, we studied ubiquitin-Proteasome system the effects of predator abundance and habitat availability on the abundance of the threespot damselfish Stegastes planifrons, a species that creates algal gardens at the expense of live coral cover. Across 21 sites,

predator biomass ranged from 12 to 193 g m(-2) (mean = 55.1; SD = 49.1) and benthic cover of S. planifrons’ preferred habitat (corals of the Orbicella species complex) ranged from 2.2 to 38.0% (mean = 14.3; SD = 9.6). Across these gradients, the local abundance of S. planifrons was significantly and negatively related to predator biomass, but not to habitat availability. Increased local abundance of S. Compound C planifrons corresponded to an increasingly larger proportion of coral colonies affected by its ‘farming behavior’, resulting in an increased prevalence of coral disease. Thus, predators indirectly affected the composition of reef communities around Bonaire by controlling damselfish abundance. Furthermore, the abundance of S. planifrons could not be correlated with its preferred habitat, despite such correlations

having been observed

elsewhere in the Caribbean.”
“To engineer a multifunctional xylan-degrading enzyme, a chimera was created by fusing the xylanase domain of the Clostridium thermocellum xylanase (xynZ) and a dual functional arabinofuranosidase/xylosidase (DeAFc; from a compost starter mixture) through a flexible peptide linker. The xylanase domain Epigenetics inhibitor of xynZ possesses previously unreported endoglucanase activity. The chimera, possessing the activities of xylanase, endoglucanase, arabinofuranosidase and xylosidase, was expressed in E. coli and purified. The chimera closely resembled the parental enzymes in pH, temperature optima and kinetics, and was more active than the parental enzyme mixture in the hydrolysis of natural xylans and corn stover.”
“To develop a technique of obtaining monoclinic polymorph of paracetamol suitable for direct compression without excipients.\n\nPreparation of spongy monoclinic paracetamol was based on quench-cooling of paracetamol solutions in water-acetone mixtures sprayed into a vessel with liquid nitrogen followed by removal of solvents by freeze-drying. X-ray powder diffraction was used to study annealing of quench-cooled solutions in “paracetamol-acetone-water” and “acetone-water” systems and to find optimum conditions for obtaining fine particles of pure monoclinic paracetamol. Samples were characterized by electron microscopy; compression properties were measured.

Further steps involved the oxidative opening of the aromatic ring, leading to carboxylic acids and inorganic ions (C) 2010 Elsevier B V All rights reserved.”
“It

has been widely reported that colloids can travel faster and over longer distances in natural structured porous media than in uniform structureless media used in laboratory studies. The presence of preferential pathways YM155 for colloids in the subsurface environment is of concern because of the increased risks for disease caused by microorganisms and colloid-associated contaminants. This study presents a model for colloid transport in dual-permeability media that includes reversible and irreversible retention of colloids and first-order exchange between the aqueous phases of the two regions. The model may also be used to describe transport of other reactive solutes in Acalabrutinib inhibitor dual-permeability media. Analytical solutions for colloid concentrations in aqueous and solid phases were obtained using Laplace transformation and matrix decomposition. The solutions proved convenient to assess the effect of model parameters on the colloid distribution. The analytical model was used to describe effluent concentrations for a bromide tracer

and 3.2- or 1-mu m-colloids that were observed after transport through a composite 10-cm long porous medium made up of a cylindrical lens or core of sand and a surrounding matrix with sand of a different grain size. The tracer data were described very well and realistic estimates were obtained for the pore-water velocity in the two flow domains. An accurate description was also achieved for most colloid breakthrough curves. Dispersivity and retention parameters were typically greater for the larger 3.2-mu m-colloids while both reversible and irreversible retention rates tended to be higher for the finer sands than the coarser sand. The relatively small sample size and the complex flow pattern in the composite medium made www.selleckchem.com/products/ABT-263.html it difficult to reach definitive conclusions regarding

transport parameters for colloid transport. (C) 2013 Elsevier B.V. All rights reserved.”
“Mesothelioma usually leads to death within 8-14 months of diagnosis. To increase the potency of oncolytic measles viruses (MVs) for mesothelioma therapy, we inserted the interferon b (IFN beta) gene alone or with the human thyroidal sodium iodide symporter (NIS) gene into attenuated MV of the Edmonston lineage. The corresponding mouse IFNb (mIFN beta) viruses, MV-mIFN beta and MV-mIFN beta-NIS, successfully propagated in human mesothelioma cells, leading to intercellular fusion and cell death. High levels of mIFNb were detected in the supernatants of the infected cells, and radioiodine uptake was substantial in the cells infected with MV-mIFNb-NIS.

coelicolor cell walls. Phenotypic analyses revealed that selleck these enzymes are important throughout development; deletion of each hydrolase gene resulted in a mutant strain that was heat sensitive, defective in spore formation, and either altered in vegetative growth or delayed in spore germination. Our results indicate that these enzymes play key roles at multiple stages in the growth and

development of S. coelicolor, highlighting both the lack of redundancy in hydrolase activity and the importance of cell wall remodeling in the S. coelicolor life cycle.”
“Due to the overexpression of a folate receptor (FR) on many malignant cells, folate-targeted drugs have been developed to improve the cancer specificity of chemotherapeutic agents. Therapeutic index is further enhanced with the use of self-immolative linkers that efficiently release the attached drug

upon cellular internalization of the folate-drug conjugate. Because FR is also abundant in normal kidney proximal tubule (PT) cells, we sought to examine in real time the trafficking and release of folate-targeted drugs in the kidney in vivo. Thus, we conducted two-photon kidney imaging studies in mice utilizing a Forster resonance energy transfer (FRET) based folate conjugate that undergoes a color shift from red to green upon reduction of the disulfide bond linking folate to a surrogate drug molecule. Following infusion via intravenous injection, folate-FRET reached the kidney in its intact unreduced form. The folate-FRET conjugate was then filtered into the lumen of PT, where it was efficiently captured by FR. As FR transcytosed across

PT, some disulfide reduction occurred, with Milciclib reduced folate-FRET detectable in PT vesicles 30 min postinjection. Prolonged monitoring of folate-FRET in mice showed modest progression of reduction in PT cells over time. Moreover, inhibition of FR trafficking in PT cells by colchicine did not significantly affect the rate or extent of folate-FRET reduction. Finally, the lack of cytosolic accumulation of released drug surrogate in the PT suggests that drug release via disulfide bond reduction ERK inhibitor should cause little kidney toxicity.”
“Objective: To investigate the sleep/wake, day/night, and 24-h periodicity of pediatric evolution to generalized tonic-clonic seizures (GTC).\n\nMethods: Charts of 407 consecutive patients aged 0-21 years undergoing continuous video-EEG monitoring for epilepsy were reviewed for the presence of GTC evolution. Seizures were characterized according to 2001 ILAE terminology. Charts were reviewed for EEG seizure localization, MRI lesion, and for seizure occurrence in 3-h time blocks, out of sleep or wakefulness, and during the day (6 AM-6 PM) or night. Analysis was done with binomial testing. Regression models were fitted using generalized estimating equations with patients as the cluster level variable.\n\nResults: 71 patients (32 girls, mean age 12.63 +/- 5.

1 (95% CI 270.4, 271.1) for cases compared to 274.7 (95% CI 274.4, 275.1)

days for controls (p < 0.0001). The odds of early term birth was increased by threefold in cases (OR 3.28; 95% CI 2.60-4.15; p < 0.0001). The mean birth weight in cases (3285.3 g) (95% CI 3242.6, 3327.9) was lower than the controls, 3373.8 g (95% CI 3348.9, 3398.7) (p = 0.0004). Maternal IL-1 beta was greater in cases (22.8 ng/ml; range 5.2-157.7 ng/ml) compared to controls (5.7; range 2.4-69.5 ng/ml; p < Linsitinib ic50 0.0001). IL-1 beta was higher in fetal plasma in cases vs. controls (20.33 vs. 8.18 ng/ml; p = 0.01). A 10 ng/ml increase in maternal IL-1 beta was associated with increased risk for GBS infection (OR:

1.628, CI: 1.163-2.278; p = 0.0045). GBS colonization shortened gestational age at term and IL-1 beta concentration in maternal plasma is an indicator of GBS status.”
“Methods: A group of 27 patients with SSc were subjected Dinaciclib inhibitor to standard electrocardiography (ECG) examination and 24-hour Holter monitoring. Analysis of HRV in time and frequency domains, HRT, and echocardiography were also performed.\n\nResults: Holter monitoring revealed a larger number of premature supraventricular contractions (PSVCs), as well as premature ventricular contractions (PVCs) in the patients with systemic scleroderma, as compared with the control group. Moreover, the SSc patients showed decreased parameters of time and frequency domains, as referred to the controls, especially during night hours. In four patients, abnormal HRT

values were present. On echocardiography, only slight changes were found, however in five patients left ventricle diastolic dysfunction was diagnosed.\n\nConclusions: The noninvasive electrocardiographic methods seems to be useful for detecting early heart involvement in course of SSc and could be recommended for routine used in clinical practice. Significance of HRT STA-9090 solubility dmso analysis in patients with SSc needs further elucidation.\n\nAnn Noninvasive Electrocardiol 2009;14(3):251-257.”
“The systemic investigation of the structural impacts of side chains on the pH- and thermo-responsiveness of tertiary amine functionalized poly(l-glutamate)s (TA-PGs) was carried out. The TA-PGs polymers were effectively synthesized by Cu(I)-catalyzed azide-alkyne cycloaddition click reaction of azido tertiary amines with poly(-propargyl-l-glutamate) (PPLG). Turbimetric measurements were performed to characterize the pH- and temperature-induced phase transition of TA-PGs in aqueous solution, which suggested a structural dependence of the properties on the N-substituted groups and the linkers between 1,2,3-triazole ring and the tertiary amine groups in the side chains.

To investigate this genotype-phenotype incongruence of “low-MIC vancomycin-resistant enterococci” (LM-VRE), we examined the molecular characteristics of these isolates, including the presence of the vanB operon, using PCR amplification and DNA sequencing. All LM-VRE isolates contained vanB associated with the transposon Tn1549 and were polyclonal. Sequencing of the vanB ligase gene showed no differences from the prototype vanB2. In addition, we examined supplemented

media to improve phenotypic detection of these isolates. Etest detection of LM-VRE improved when Mueller-Hinton agar (MHA) and brain heart infusion agar (BHIA) were supplemented with 10 g/liter oxgall (MHA-Oxg and BHIA-Oxg, respectively).

We assessed the sensitivity and specificity of these Volasertib media to detect vancomycin resistance using vancomycin-resistant vanB-containing E. faecium (n = 11), vancomycin-susceptible 4SC-202 (van negative) E. faecium (n = 11), vancomycin-susceptible (van negative) E. faecalis (n = 11), and our LM-VRE (n = 23) isolates. After 48 h of incubation, both MHA-Oxg and BHIA-Oxg were 100% (34/34) sensitive and 100% (22/22) specific in the identification of vancomycin resistance. These findings suggest that supplementation of MHA or BHIA with 10 g/liter oxgall should be considered in laboratories where VRE detection protocols rely primarily on strain phenotype rather than early vanB gene detection by PCR.”
“Community-associated methicillin-resistant Staphylococcus aureus Smoothened Agonist cost (CA-MRSA) has recently emerged worldwide. The United States, in particular, is experiencing a serious

epidemic of CA-MRSA that is almost entirely caused by an extraordinarily infectious strain named USA300. However, the molecular determinants underlying the pathogenic success of CA-MRSA are mostly unknown. To gain insight into the evolution of the exceptional potential of USA300 to cause disease, we compared the phylogeny and virulence of USA300 with that of closely related MRSA clones. We discovered that the sublineage from which USA300 evolved is characterized by a phenotype of high virulence that is clearly distinct from other MRSA strains. Namely, USA300 and its progenitor, USA500, had high virulence in animal infection models and the capacity to evade innate host defense mechanisms. Furthermore, our results indicate that increased virulence in the USA300/USA500 sublineage is attributable to differential expression of core genome-encoded virulence determinants, such as phenol-soluble modulins and alpha-toxin. Notably, the fact that the virulence phenotype of USA300 was already established in its progenitor indicates that acquisition of mobile genetic elements has played a limited role in the evolution of USA300 virulence and points to a possibly different role of those elements.

\n\nResults: The mean baseline AHI was reduced with an oral appliance from 21 +/- 4 to 9.8 +/- 8 in 95 subjects. Thirty-five patients were regarded as responders. Logistic regression analyses revealed that both MS and BMI could individually predict the treatment outcome. When the cutoff value of BMI was determined to be 24 kg/m(2) based on a receiver operating characteristic curve, 53 obese patients (ie, BMI >24 kg/m(2)) with an MS of class 4 were indicative of treatment failure with a high negative predictive value (92) and a low negative likelihood ratio (0.28).\n\nConclusions:

We conclude that patients with moderate OSA who are obese with oropharyngeal crowding are unlikely to respond to oral appliance treatment. This simple prediction GSK2126458 mw can be applied without the need for any cumbersome tools immediately after the diagnosis of OSA.”
“Collagen V, a fibrillar collagen buy Autophagy inhibitor with important functions in tissues, assembles into distinct chain associations. The most abundant and ubiquitous molecular

form is the heterotrimer [alpha 1(V)] (2)alpha 2(V). In the attempt to produce high levels of recombinant collagen V heterotrimer for biomedical device uses, and to identify key factors that drive heterotrimeric chain association, several cell expression systems (yeast, insect, and mammalian cells) have been assayed by cotransfecting the human pro alpha 1(V) and pro alpha 2(V) chain cDNAs. Suprisingly, in all recombinant expression systems, the formation of [alpha 1(V)](3) homotrimers was considerably favored over the heterotrimer. In addition, see more pepsin-sensitive

pro alpha 2(V) chains were found in HEK-293 cell media indicating that these cells lack quality control proteins preventing collagen monomer secretion. Additional transfection with Hsp47 cDNA, encoding the collagen-specific chaperone Hsp47, did not increase heterotrimer production. Double immunofluorescence with antibodies against collagen V alpha-chains showed that, contrary to fibroblasts, collagen V alpha-chains did not colocalized intracellularly in transfected cells. Monensin treatment had no effect on the heterotrimer production. The heterotrimer production seems to require specific machinery proteins, which are not endogenously expressed in the expression systems. The different constructs and transfected cells we have generated represent useful tools to further investigate the mechanisms of collagen trimer assembly.”
“An extractive spectrophotometric analytical method has been developed for the determination of uranium in ore leach solution. This technique is based on the selective extraction of uranium from multielement system using a synergistic mixture of 2-ethylhexyl phosphonic acid-mono-2-ethylhexyl ester (PC88A) and tri-n-octyl phosphine oxide (TOPO) in cyclohexane and color development from the organic phase aliquot using 2-(5-Bromo-2-pyridylazo)-5-diethyl aminophenol (Br-PADAP) as chromogenic reagent.